High-quality monoclonal Antibody Humanization Service and AI-Powered Antibody Affinity Maturation Service
Recombinant Proteins | Antibodies | cDNA/genes/qPCR | ELISA | Viral Antigens & Pathogens | Immunodiagnostic | Organoid & Stem Cell Reagents
By using complementarity-determining region (CDR) grafting and computational molecular modeling, Sino Biological is able to provide monoclonal antibody humanization service with a high humanization rate (>95%). Sino Biological also offers an AI-enabled platform for antibody affinity maturation, which can effectively rank up to 1010 antibody sequences based on their predicted affinity to one or more antigens within a few days. It helps to quickly obtain high-affinity antibodies and speed up the drug development processes.
| Item | Description | Timeline | Deliverable |
|---|---|---|---|
| Antibody humanization | Affinity validation | 3-4 weeks | • Top 3 humanized antibodies, 0.5 mg/clone (at least 1 humanized Ab with affinity comparable with parental Ab) • Antibody sequences • Plasmids containing H and L chains • CoA |
| Mouse antibody/antigen affinity validation | |||
| Preparation of human-mouse chimeric antibody | |||
| • Transient expression and purification • Affinity validation | |||
| Preparation of humanized variants | |||
| • 3D structural modeling of antibody • Back mutation | |||
| • Transient expression and purification • Affinity validation | |||
| Antibody affinity maturation | Mutagenesis Library Generation | 4-6 weeks | • Top 3 humanized antibodies, 0.5 mg/clone (at least 1 humanized Ab with affinity comparable with parental Ab) • Antibody sequences • Plasmids containing H and L chains • CoA |
| AI-Powered Antibody Affinity Prediction | |||
| Transient expression and purification | |||
| Affinity validation |
The humanized antibody has comparable affinity with the parental antibody
Affinity increase from 10-9 to 10-11, up to 218 fold higher
Bio-Connect Life Sciences supplies Sino Biological products and services in the Benelux. If you have any questions, please contact the helpdesk.
Recombinant Proteins | Antibodies | cDNA/genes/qPCR | ELISA | Viral Antigens & Pathogens | Immunodiagnostic | Organoid & Stem Cell Reagents
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