15 July 2025

OriGene’s Custom Protein Services: Made in the US

OriGene’s custom protein services offer tailored solutions for every research need, from gene cloning to protein purification.

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Genomics & Proteomics | cDNA Clones | Empty Vectors | Viral Gene Delivery | LV & AAV Vectors | RNAi | cloning | CRISPR | antibodies | IHC | ELISA | Proteins | Tissues

With over 25 years of experience, OriGene offers comprehensive custom protein services to accelerate your research, whether you’re targeting a novel protein or need a customized solution from our collection of over 19,000 recombinant proteins. As a trusted partner in end-to-end protein development, our capabilities span the full workflow—from gene cloning and protein expression to high-quality protein purification. We specialize in expressing challenging targets and offer flexible expression systems tailored to your specific project needs, supporting both basic research and therapeutic discovery.

Features

  • Success Stories: 2,000+ projects | 500+ satisfied customers
  • Multiplex Expression Systems: Choose from mammalian (HEK293T or CHO) and bacterial (E. coli) expression systems
  • Scalable: Whether the need is small or large, we offer protein services from μg to gm levels
  • Tag Customization: Customize your protein based on your application – Tag-free or add N- or C-fusion tags
  • Site-specific Biotinylation: Modify your protein of interest with site-specific biotinylation using birA.
  • Additional Customization: We offer additional customizations, including mutant protein expression, buffer customization, SEC purification, and more.
  • Transparent pricing: Invoiced only for the successful completion of milestones
  • Made in the USA: Our custom proteins are proudly manufactured here in Rockville, Maryland

Case Studies

1. Expression Systems

2. Site-specific Biotinylation

SDS-PAGE and Western verified site-specific biotinylated PKP2 human recombinant protein

Mono-biotinylated via Avi-tag

Biotin to protein ratio of 1:1 as determined by streptavidin pull down assay

3. Custom Modification

KRAS mutant (G12D) Myc-DDK tagged human recombinant protein
Tagged and Tag free GALT human recombinant protein

Available Custom Recombinant Protein Expression Systems

Mammalian Expression System

Common ApplicationsAdvantagesChallenges
• Functional and structural analysis

• Antibody production

• Protein interactions

• Production of enzyme in large scale

• Virus production
• Most authentic human protein products

• Best preservation of native structure, function, and post-translational modifications

• Create stable production cell lines for scalable solutions
• Complex culture conditions

• Costly set-up and maintenance

• Low yields

• Scaling difficulties

Insect Expression System

Common ApplicationsAdvantagesChallenges
• Functional and structural analysis

• Expression of protein complexes

• Expression of intracellular proteins

• Virus-like particles production
• Post-translation modifications profile is similar to mammalian cells

• Effective protein folding

• More scalable than mammalian cells
• Virus production can be time consuming

• Tricky to culture

• Infection can induce premature lysis

Bacterial Expression System

Common ApplicationsAdvantagesChallenges
• Structural analysis

• Antibody production

• Protein interactions

• Enzyme production

• Vaccine development
• Simple vector construction

• Straightforward culture methods

• High yields

• Amenable to scaling
• No post-translation modifications

• Improperly folded product

• Susceptible to aggregation

• Potential endotoxin contamination

Interested in Custom Protein Expression Solutions?

For more information about Origene’s custom recombinant protein expression systems, please contact one of our account managers.

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Genomics & Proteomics | cDNA Clones | Empty Vectors | Viral Gene Delivery | LV & AAV Vectors | RNAi | cloning | CRISPR | antibodies | IHC | ELISA | Proteins | Tissues

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