Neurodegenerative Disease Markers

Alzheimer’s disease (AD), Parkinson’s disease (PD), and other neurodegenerative diseases are complex and prescient subjects of research, characterized by the accumulation of misfolded proteins in the nervous system that exhibit pathogen behavior (Armstrong, 2020). With as many as 55 million people with AD or dementia and an estimated 8.5 million people with PD worldwide, and those numbers expected to rise, the need for comprehensive understanding of the pathways that contribute to neurodegenerative pathogenesis is only growing.

To aid in the specific, reliable detection and modulation of proteins like polysialic acid, amyloid beta, tau, and more, Absolute Antibody recombinantly engineers antibodies against relevant proteins implicated in neurodegenerative disease. Recent citations in the field of neurodegenerative disease research illustrate our antibodies in action.

Neurodegenerative Disease Antibodies in Action

• The anti-amyloid beta clone 6E10 in the proprietary AbFab2™ recombinant F(ab2) format (Ab00714-1.7) was used for immunoprecipitation assays in this 2019 Molecular Cell study to investigate the role of a protein complex called the nascent polypeptide-associated complex (NAC) in helping prevent protein misfolding, which is linked to diseases like Huntington’s and spinocerebellar ataxias. The researchers found that NAC can assist in the proper folding of various proteins, suggesting that NAC could be a promising therapeutic target to prevent protein misfolding diseases.
• In this Frontiers in Cellular Neuroscience publication, researchers use the anti-amyloid beta [WO2] antibody engineered into the mouse IgG2a format (Ab00239-2.0) in western blot analysis of rat brain tissues as part of a methodology to mimic Alzheimer’s disease (AD) progression in rat models. This methodology adds to the tools available to explore and manipulate the variety of pathways that are a part of AD progression, broadening our understanding of this neurodegenerative disease.
• Researchers studied polysialation, a posttranslational modification implicated in processes like learning and neurodegenerative disease, in different brain regions of mice across their estrous cycle in this 2020 Hormones and Behavior article. Researchers performed immunohistochemical staining for polysialic acid on different regions of the mouse brain at different stages of estrous using our recombinant antibody against polysialic acid [735] (Ab00240) as the primary antibody. The results suggest that polysialic acid immunoreactivity differs across the mouse estrous cycle in brain sub-regions that affect behavior.

Recombinant Engineered Antibodies

The Absolute Antibody catalog offers recombinantly produced antibodies against relevant proteins in neurodegenerative disease research, like polysialic acid (Ab00240), amyloid beta (Ab00714 and Ab00239), and tau proteins (Ab00639) to ensure batch-to-batch reproducibility and enable antibody engineering. The antibodies are available off-the-shelf in different engineered formats, including mouse, rabbit and human species, as well as various isotypes and fragments. The antibodies can also be custom engineered into other formats upon request

Further, Absolute Antibody confirmed the specificity of the recombinantly produced clones. The figure below illustrates the purity (A), specificity (B, C), and activity (D, E) of our recombinant WO2 anti-Amyloid beta (Ab00239-2.0) antibody compared to a hybridoma-derived WO2 from Competitor M. More information can be found in our post about how recombinant amyloid beta antibodies support Alzheimer’s research.

If you can’t find what you need in our full catalog of neuroscience research antibodies, our antibody engineers can tailor any antibody or sequence to exactly what you need. Our custom services offerings include antibody engineering in every format, optimized antibody expression via our recombinant system, and antibody sequencing to protect your work. Please contact us for more information.

Absolute Antibody offers neurodegenerative disease research reagents from other brands within our parent company Absolute Biotech: goat polyclonal antibodies from Everest Biotech; antibodies and cell lines from Kerafast; and antibodies, proteins and ELISA kits from LSBio.