D-ACF Media: Setting a new standard in fibroblast culture

Recent advances in dermatological research demand more reliable, ethical, and reproducible methods for dermal fibroblast culture. PromoCell’s Fibroblast Growth Medium D-ACF addresses these needs by offering an unparalleled solution: a defined and animal component-free formulation that provides superior results.

Designed with precision, safety, and ethical considerations, this innovative medium ensures outstanding performance in cultivating normal human dermal fibroblasts (NHDF) from both juvenile and adult donors. Its advanced formulation allows direct plating on cell culture-treated plastic, eliminating the need for extracellular matrix (ECM) coating and streamlining your research workflow.

Discover Fibroblast Growth Medium D-ACF (C-23027)

Key advantages:

• Exceptional standardization and reproducibility through a meticulously optimized, defined, and animal-component-free formulation.
• Ethical compliance with zero animal components, minimizing regulatory hurdles.
• Robust cell viability, guaranteed for up to 15 population doublings in juvenile NHDF.
• Enhanced growth, providing juvenile NHDF doubling times that match or exceed those achieved with conventional media.
• Sustained marker profile, maintaining CD90 expression up to 15 population doublings.
• High flexibility, with a phenol red-free option for specialized applications tailored to your needs.
• The compatibility of the product makes it also suitable for applications involving fibroblasts from adult donors.

This pioneering growth medium empowers researchers with reliable, consistent, and ethical cell culture conditions for fibroblasts, ensuring reproducible and compliant research findings.

For greater flexibility in your fibroblast research, PromoCell’s new Fibroblast Growth Medium D-ACF also comes in a phenol red-free version. This phenol red-free formulation is perfect for specialized applications and sensitive experiments, such as fluorescence-based assays, live-cell imaging, and high-throughput screening. Additionally, it is ideal for those who wish to avoid phenol red because of concerns regarding estrogen sensitivity in their cells.

Learn more about the impact of Defined and Animal Component-Free culture media?

Please contact one of our account managers, or request a quote directly.

Access PromoCell’s latest infographic, providing a comprehensive overview of the critical aspects of defined and animal component-free (D-ACF) cell culture media and how they are transforming dermatological research. The infographic also highlights the structure of the skin and the applications of dermal fibroblasts in wound healing, hair follicle regeneration, and aging studies. In addition, the infographic summarizes research statistics related to dermal fibroblasts, including FDA-approved therapies and ongoing clinical trials.